Immunodetection was performed with corresponding horseradish peroxide-conjugated extra antibodies and detected by enhanced chemiluminescence assay (ECl, Amersham, Piscataway, NJ). 17-AAG downregulated cyclin D1, cyclin E, CDK6 and CDK4, it resulted in cyclin CDK2 and A deposition, that was reversed with the addition of U0126. Anti-proliferation assay demonstrated that mix of U0126 and 17-AAG led to … Continue reading Immunodetection was performed with corresponding horseradish peroxide-conjugated extra antibodies and detected by enhanced chemiluminescence assay (ECl, Amersham, Piscataway, NJ)